Antifreeze Proteins
Mostrando 1-12 de 19 artigos, teses e dissertações.
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1. Proteína estruturadora de gelo em cultivares brasileiras de trigo e centeio: ocorrência, caracterização e aplicação em massas congeladas / Ice structuring protein in Brazilian wheat and rye varieties: occurrence, characterization and application in frozen dough
Proteína estruturadora de gelo (ISP) têm demonstrado um grande potencial na melhoria da qualidade dos alimentos congelados, e a obtenção dessas proteínas a partir de fontes naturais tem sido considerada importante. Cultivaress de trigo de inverno (IPR 84 e CD 104) e primavera (BRS Guabiju, Ônix e LD 062212) e centeio (IPR 89) após 12 dias de germinaç
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/03/2011
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2. Ethylene Induces Antifreeze Activity in Winter Rye Leaves1
Antifreeze activity is induced by cold temperatures in winter rye (Secale cereale) leaves. The activity arises from six antifreeze proteins that accumulate in the apoplast of winter rye leaves during cold acclimation. The individual antifreeze proteins are similar to pathogenesis-related proteins, including glucanases, chitinases, and thaumatin-like proteins
American Society of Plant Physiologists.
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3. Antifreeze proteins in winter rye are similar to pathogenesis-related proteins.
The ability to control extracellular ice formation during freezing is critical to the survival of freezing-tolerant plants. Antifreeze proteins, which are proteins that have the ability to retard ice crystal growth, were recently identified as the most abundant apoplastic proteins in cold-acclimated winter rye (Secale cereale L.) leaves. In the experiments r
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4. Extraction and Isolation of Antifreeze Proteins from Winter Rye (Secale cereale L.) Leaves.
Apoplastic extracts of cold-acclimated winter rye (Secale cereale L. cv Musketeer) leaves were previously shown to exhibit antifreeze activity. The objectives of the present study were to identify and characterize individual antifreeze proteins present in the apoplastic extracts. The highest protein concentrations and antifreeze activity were obtained when t
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5. Snow-Mold-Induced Apoplastic Proteins in Winter Rye Leaves Lack Antifreeze Activity1
During cold acclimation, winter rye (Secale cereale L.) plants secrete antifreeze proteins that are similar to pathogenesis-related (PR) proteins. In this experiment, the secretion of PR proteins was induced at warm temperatures by infection with pink snow mold (Microdochium nivale), a pathogen of overwintering cereals. A comparison of cold-induced and patho
American Society of Plant Physiologists.
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6. Calcium Interacts with Antifreeze Proteins and Chitinase from Cold-Acclimated Winter Rye1
During cold acclimation, winter rye (Secale cereale) plants accumulate pathogenesis-related proteins that are also antifreeze proteins (AFPs) because they adsorb onto ice and inhibit its growth. Although they promote winter survival in planta, these dual-function AFPs proteins lose activity when stored at subzero temperatures in vitro, so we examined their s
American Society of Plant Biologists.
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7. Immunolocalization of Antifreeze Proteins in Winter Rye Leaves, Crowns, and Roots by Tissue Printing.
During cold acclimation, antifreeze proteins (AFPs) that are similar to pathogenesis-related proteins accumulate in the apoplast of winter rye (Secale cereale L. cv Musketeer) leaves. AFPs have the ability to modify the growth of ice. To elucidate the role of AFPs in the freezing process, they were assayed and immunolocalized in winter rye leaves, crowns, an
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8. DNA sequence coding for an antifreeze protein precursor from winter flounder.
A cDNA made to antifreeze protein mRNA of the winter flounder was cloned in the plasmid pBR322 and its sequence was determined by the method of Maxam and Gilbert. Its sequence codes for a precursor protein that is 82 amino acids in length. This precursor has both a signal polypeptide and a prosequence before the mature protein of 38 amino acid residues. The
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9. Evolution of antifreeze glycoprotein gene from a trypsinogen gene in Antarctic notothenioid fish
Freezing avoidance conferred by different types of antifreeze proteins in various polar and subpolar fishes represents a remarkable example of cold adaptation, but how these unique proteins arose is unknown. We have found that the antifreeze glycoproteins (AFGPs) of the predominant Antarctic fish taxon, the notothenioids, evolved from a pancreatic trypsinoge
The National Academy of Sciences of the USA.
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10. Chitinase Genes Responsive to Cold Encode Antifreeze Proteins in Winter Cereals1
Antifreeze proteins similar to two different chitinases accumulate during cold acclimation in winter rye (Secale cereale). To determine whether these cold-responsive chitinases require post-translational modification to bind to ice, cDNAs coding for two different full-length chitinases were isolated from a cDNA library produced from cold-acclimated winter ry
American Society of Plant Physiologists.
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11. Antifreeze glycoproteins inhibit leakage from liposomes during thermotropic phase transitions.
Antifreeze glycoproteins (AFGPs), found in the blood of polar fish at concentrations as high as 35 g/liter, are known to prevent ice crystal growth and depress the freezing temperature of the blood. Previously, Rubinsky et al. [Rubinsky, B., Mattioli, M., Arav, A., Barboni, B. & Fletcher, G. L. (1992) Am. J. Physiol. 262, R542-R545] provided evidence that AF
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12. Cloning and Expression of afpA, a Gene Encoding an Antifreeze Protein from the Arctic Plant Growth-Promoting Rhizobacterium Pseudomonas putida GR12-2†
The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was cloned by PCR and inverse PCR using primers d
American Society for Microbiology.