5-Bromo-2′-deoxyuridine Potentiation of Transformation of Rat-Embryo Cells Induced In Vitro by 3-Methylcholanthrene: Induction of Rat Leukemia Virus gs Antigen in Transformed Cells
AUTOR(ES)
Freeman, Aaron E.
RESUMO
Low-passage rat-embryo cells were not transformed by 3-methylcholanthrene or by 5-bromo-2′ deoxyuridine. However, prior treatment with bromodeoxyuridine, followed by treatment with methylcholanthrene, resulted in cell transformation about three subpassages after removal of the carcinogen. RNA-directed DNA polymerase activity could not be detected in either normal or transformed cells. However, gs-1 antigen specific for rat C-type RNA virus was detected in cultures derived from bromodeoxyuridine-treated cells. No gs-1 antigen for the C-type RNA virus was detected in cultures that had not been treated with bromodeoxyuridine during the 25 subpassages of these experiments.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433747Documentos Relacionados
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