5'-Terminal 7-methylguanosine and mRNA function: influence of potassium concentration on translation in vitro.
AUTOR(ES)
Weber, L A
RESUMO
Vaccinia mRNAs containing either 5'-terminal m7G or unmethylated 5'-terminal structures were synthesized in vitro and their relative efficiencies of translation were compared in wheat germ and reticulocyte cell-free protein-synthesizing systems. The importance of the m7G group for efficient translation increases as the K+ concentration is raised. At K+ concentrations optimal for translation of mRNA containing m7G, unmethylated mRNA is translated at the same relative low efficiency in both cell extracts. The rate of binding of mRNA to ribosomes at K+ concentrations close to those found in intact cells is strongly influenced by the presence of m7G regardless of the source of the cell extract.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=431520Documentos Relacionados
- 5′-Terminal 7-methylguanosine and mRNA function: Influence of potassium concentration on translation in vitro
- Influenza viral mRNA contains internal N6-methyladenosine and 5'-terminal 7-methylguanosine in cap structures.
- Requirement for 7-methylguanosine in translation of globin mRNA in vivo.
- Terminal 7-methyl-guanosine cap structure on the normally uncapped 5' noncoding region of poliovirus mRNA inhibits its translation in mammalian cells.
- In vitro selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNA molecules