A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae.
AUTOR(ES)
Cottarel, G
RESUMO
Saccharomyces cerevisiae centromeres contain a conserved region ranging from 111 to 119 base pairs (bp) in length, which is characterized by the three conserved DNA elements CDEI, CDEII, and CDEIII. We isolated a 125-bp CEN6 DNA fragment (named ML CEN6) containing only these conserved elements and assayed it completely separated from its chromosomal context on circular minichromosomes and on a large linear chromosome fragment. The results show that this 125-bp CEN6 DNA fragment is by itself sufficient for complete mitotic and meiotic centromere functions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=362379Documentos Relacionados
- Single base-pair mutations in centromere element III cause aberrant chromosome segregation in Saccharomyces cerevisiae.
- Centromere DNA mutations induce a mitotic delay in Saccharomyces cerevisiae.
- Mutational Analysis of Meiotic and Mitotic Centromere Function in Saccharomyces cerevisiae
- An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae.
- A 15-base-pair element activates the SPS4 gene midway through sporulation in Saccharomyces cerevisiae.
