A 69-base-pair monkey DNA sequence enhances simian virus 40 replication and transcription through multiple motifs.
AUTOR(ES)
Szymanski, P
RESUMO
We have determined that a 69-base-pair (bp) monkey DNA sequence, previously found to enhance simian virus 40 replication, has transcriptional enhancer activity as well. Consensus recognition sites for the transcription factor AP-1, present at each end of this sequence, are partially responsible for its replication- and transcription-enhancing activities. Other motifs within the 69-bp monkey sequence also act to increase the levels of replication and transcription. The activity of the monkey sequence is augmented by the presence of a simian virus 40 21-bp repeat. The 69-bp sequence enhances transcription but not replication from a distance. We conclude that the stimulation of replication and transcription can be uncoupled, suggesting that different mechanisms may be involved.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=249257Documentos Relacionados
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