A 96-well epifluorescence assay for rapid assessment of compounds inhibitory to Candida spp.
AUTOR(ES)
Hector, R F
RESUMO
A rapid method for the screening of potential antifungal compounds was developed. A variety of compounds were tested against regenerating protoplasts of Candida spp. in a microtiter format. The degree of cell wall formation was assessed by staining with Cellufluor (Polysciences, Inc., Warrington, Pa.), a fluorochrome with known affinity for chitin, followed by determination of fluorescence by using a Dynatech Microfluor reader (Dynatech Laboratories, Inc., Alexandria, Va.). Compounds with known activity against the cell wall or cytoplasmic membrane of fungi inhibited wall synthesis in a concentration-dependent fashion. Treatment with 5-fluorocytosine, however, resulted in no inhibition. In general, protoplasts of C. albicans regenerated more quickly and were more sensitive to the compounds tested than protoplasts of C. tropicalis and C. parapsilosis. While the described method is not specific for a given class of antifungal agents, it may prove useful for testing large numbers of compounds quickly.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=268983Documentos Relacionados
- Microbiological assay for vitamin B12 performed in 96-well microtitre plates.
- Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing.
- Mini-transfections on 96-well microtiter plates.
- Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp.
- Integrative modification of YAC clones in a 96-well microplate format.