A cell-counting factor regulating structure size in Dictyostelium
AUTOR(ES)
Brock, Debra A.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
Developing Dictyostelium cells form large aggregation streams that break up into groups of 0.2 × 105 to 1 × 105 cells. Each group then becomes a fruiting body. smlA cells oversecrete an unknown factor that causes aggregation streams to break up into groups of ∼5 × 103 cells and thus form very small fruiting bodies. We have purified the counting factor and find that it behaves as a complex of polypeptides with an effective molecular mass of 450 kD. One of the polypeptides is a 40-kD hydrophilic protein we have named countin. In transformants with a disrupted countin gene, there is no detectable secretion of counting factor, and the aggregation streams do not break up, resulting in huge (up to 2 × 105 cell) fruiting bodies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=316923Documentos Relacionados
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