A charged residue at the subunit interface of PCNA promotes trimer formation by destabilizing alternate subunit interactions
AUTOR(ES)
Freudenthal, Bret D.
FONTE
International Union of Crystallography
RESUMO
Eukaryotic proliferating cell nuclear antigen (PCNA), an essential accessory factor in DNA replication and repair, is a ring-shaped homotrimer. A novel nontrimeric structure of E113G-mutant PCNA protein is reported, which shows that this protein forms alternate subunit interactions. It is concluded that the charged side chain of Glu113 promotes normal trimer formation by destabilizing these alternate subunit interactions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2685733Documentos Relacionados
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