A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
AUTOR(ES)
Qiu, Shanlian, Chen, Jichen, Lin, Si, Lin, Xinjian
FONTE
Brazilian Journal of Microbiology
DATA DE PUBLICAÇÃO
2012-06
RESUMO
Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
Documentos Relacionados
- Comparison of Two Silver Staining Techniques for Detecting Lipopolysaccharides in Polyacrylamide Gels
- Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in stools from diarrheic patients in Thailand.
- Modification of the silver staining technique to detect lipopolysaccharide in polyacrylamide gels.
- Comparison of three staining methods for detecting microsporidia in fluids.
- Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile.
