A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes.
AUTOR(ES)
Lofquist, A K
RESUMO
We have studied the mechanism by which 5'-flanking sequences modulate the in vitro transcription of eucaryotic tRNA genes. Using deletion and linker substitution mutagenesis, we have found that the 5'-flanking sequences responsible for the different in vitro transcription levels of three Drosophila tRNA5Asn genes are contained within a discrete region centered 22 nucleotides upstream from the transcription initiation site. In conjunction with the A-box intragenic control region, this upstream transcription-modulatory region functions in the selection mechanism for the site of transcription initiation. Since the transcription-modulatory region directs the position of the start site and the actual sequence of the transcription-modulatory region determines the level of tRNAAsn gene transcription, the possibility is raised that the transcription-modulatory region directs a transcription initiation event similar to open complex formation at procaryotic promoters.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=365518Documentos Relacionados
- The nucleotide sequences of two tRNAAsn genes from tobacco chloroplasts.
- Sequence of tRNAAsn gene of Saccharomyces cerevisiae.
- Alternative sites of transcription initiation upstream of the canonical cap site in human gamma-globin and beta-globin genes.
- Nucleotide sequence of the bean mitochondrial DNA region containing the tRNA(Asn) and tRNA(Tyr) genes.
- Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
