A Fast Chromatographic Method for Determination of Daidzein and Genistein in Spiked Water River Samples Using Multivariate Curve Resolution
AUTOR(ES)
Moreira, Edilene D. T., Pinto, Licarion, Gomes, Adriano A., Goicoechea, Héctor C., Araújo, Mário C. U.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2015-08
RESUMO
This work reports the development of a fast chromatographic methodology for quantitation of two phytoestrogens: daidzein (DAI), and genistein (GEN), in river water samples. The proposed method is based on high performance liquid chromatography-diode array detection (HPLC-DAD) data, and multivariate curve resolution-alternative least square (MCR-ALS) second-order calibration. Initially, the method was evaluated analyzing a synthetic validation set; prepared based on a Taguchi design. Subsequently, the method was applied to predict the concentration of the phytoestrogens in spiked river water samples, previously pre-processed by solid phase extraction (SPE). By implementation of the present chromatographic methodology, a 50% reduction in operation time was achieved (from 7.00 to 3.25 min) when compared with previous work in the literature. Precision was achieved even in the presence of non-modeled constituents and strong background. Thus, the proposed method is a rapid and robust alternative for the quantitation of studied phytoestrogens.
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