A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids
AUTOR(ES)
Gao, Xiaolian
FONTE
Oxford University Press
RESUMO
Oligonucleotide microarrays or oDNA chips are effective decoding and analytical tools for genomic sequences and are useful for a broad range of applications. Therefore, it is desirable to have synthesis methods of DNA chips that are highly flexible in sequence design and provide high quality and general adoptability. We report herein, DNA microarray synthesis based on a flexible biochip method. Our method simply uses photogenerated acid (PGA) in solution to trigger deprotection of the 5′-OH group in conventional nucleotide phosphoramidite monomers (i.e. PGA-gated deprotection), with the rest of the reactions in the synthesis cycle the same as those used for routine synthesis of oligonucleotides. The complete DNA chip synthesis process is accomplished on a regular DNA synthesizer that is coupled with a UV-VIS projection display unit for performing digital photolithography. Using this method, oDNA chips containing probes of newly discovered genes can be quickly and easily synthesized at high yields in a conventional laboratory setting. Furthermore, the PGA-gated chemistry should be applicable to microarray syntheses of a variety of combinatorial molecules, such as peptides and organic molecules.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=92522Documentos Relacionados
- Light-directed synthesis of high-density oligonucleotide arrays using semiconductor photoresists
- Light-directed 5′→3′ synthesis of complex oligonucleotide microarrays
- Blue light-directed destabilization of the pea Lhcb1*4 transcript depends on sequences within the 5' untranslated region.
- The 5′ End of the Pea Ferredoxin-1 mRNA Mediates Rapid and Reversible Light-Directed Changes in Translation in Tobacco1
- A rapid deprotection procedure for phosphotriester DNA synthesis.