A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction.
AUTOR(ES)
Duwat, P
RESUMO
An internal fragment of the recA gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. The internal 348- or 360-bp recA DNA segments from Bacillus subtilis, Clostridium acetobutylicum, Lactobacillus bulgaricus, Lactobacillus helveticus, Leuconostoc mesanteroides, Listeria monocytogenes, Staphylococcus aureus, and Streptococcus salivarus subsp. thermophilus were amplified, cloned, and sequenced. The G + C contents of the DNA from these species range from 28 to 52%. The sequences of the bacterial recA genes show strong relatedness. This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=206342Documentos Relacionados
- Degenerate oligonucleotide primers for enzymatic amplification of recA sequences from gram-positive bacteria and mycoplasmas.
- A putative regulatory gene downstream of recA is conserved in gram-negative and gram-positive bacteria.
- Glass bead transformation method for gram-positive bacteria
- General method for direct cloning of DNA fragments generated by the polymerase chain reaction.
- Improved electroporation and cloning vector system for gram-positive bacteria.
