A Genome-Wide Analysis of Blue-Light Regulation of Arabidopsis Transcription Factor Gene Expression during Seedling Development1[w]
AUTOR(ES)
Jiao, Yuling
FONTE
The American Society for Plant Biologists
RESUMO
A microarray based on PCR amplicons of 1,864 confirmed and predicted Arabidopsis transcription factor genes was produced and used to profile the global expression pattern in seedlings, specifically their light regulation. We detected expression of 1,371 and 1,241 genes in white-light- and dark-grown 6-d-old seedlings, respectively. Together they account for 84% of the transcription factor genes examined. This array was further used to study the kinetics of transcription factor gene expression change of dark-grown seedlings in response to blue light and the role of specific photoreceptors in this blue-light regulation. The expression of about 20% of those transcription factor genes are responsive to blue-light exposure, with 249 and 115 genes up or down-regulated, respectively. A large portion of blue-light-responsive transcription factor genes exhibited very rapid expression changes in response to blue light, earlier than the bulk of blue-light-regulated genes. This result suggests the involvement of transcription cascades in blue-light control of genome expression. Comparative analysis of the expression profiles of wild type and various photoreceptor mutants demonstrated that during early seedling development cryptochromes are the major photoreceptors for blue-light control of transcription factor gene expression, whereas phytochrome A and phototropins play rather limited roles.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=300705Documentos Relacionados
- Genome-Wide ORFeome Cloning and Analysis of Arabidopsis Transcription Factor Genes1[w]
- Blue-Light Regulation of the Arabidopsis thaliana Cab1 Gene.
- Genome-Wide Expression Analyses of Gene Regulation during Early Development of Dictyostelium discoideum
- Organ-Specific Expression of Arabidopsis Genome during Development1[w]
- Genome-Wide Analysis of Spatial Gene Expression in Arabidopsis FlowersW⃞