A genomic gene encoding the b5 rabbit immunoglobulin kappa constant region: implications for latent allotype phenomenon.
AUTOR(ES)
Emorine, L
RESUMO
We previously reported that domestic rabbits of five immunoglobulin kappa allotype strains (b4v, b4, b5, b6, and b9) harbor at least two DNA sequences that hybridize strongly to kappa constant region probes in Southern blots. One of these sequences ("type A") has been identified as encoding the constant region of the kappa 2 isotype, an immunoglobulin chain that most rabbits express only at low levels, if at all. We identified the second sequence--for rabbits of the b4 allotype--as encoding the nominal b4 kappa chain (or kappa 1 isotype), but for rabbits of other allotypes no definite identification for this "type B" sequence could be made. Here we suggest that the type B sequence in rabbits of the other domestic allotypes also encodes the nominal kappa 1 immunoglobulin chain. We show this directly for the b5 allotype; a type B sequence cloned from b5 DNA has been found to contain an apparently functional gene encoding the b5 constant region sequence. Indirect arguments suggest the corresponding conclusion for the b4v, b6, and b9 allotypes. We have considered the implications of these results for the phenomenon of "latent allotype" expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=345006Documentos Relacionados
- cDNA clone encoding a complete rabbit immunoglobulin kappa light chain of b4 allotype.
- Rabbit immunoglobulin kappa genes: structure of a germline b4 allotype J-C locus and evidence for several b4-related sequences in the rabbit genome.
- The sequences of rabbit kappa light chains of b4 and b5 allotypes differ more in their constant regions than in their 3' untranslated regions.
- Multiplicity of constant kappa light chain genes in the rabbit genome: a b4b4 homozygous rabbit contains a kappa-bas gene.
- DNA sequence of the constant gene region of the mouse immunoglobulin kappa chain.