A hairpin ribozyme inhibits expression of diverse strains of human immunodeficiency virus type 1.
AUTOR(ES)
Yu, M
RESUMO
Ribozymes have enormous potential as antiviral agents. We have previously reported that a hairpin ribozyme expressed under the control of the beta-actin promoter that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in the leader sequence can inhibit HIV-1 (pHXB2gpt) expression. For such a ribozyme in a retroviral vector delivery system to be useful in gene therapy for the treatment of HIV-1 infection, it must be able to inhibit the expression of multiple HIV-1 strains. We have now cloned this ribozyme into various regular expression vectors (including retroviral vectors) by using various gene expression control strategies. Here we show by transient transfection that inhibition of expression of diverse strains of HIV-1 can be achieved by this ribozyme expressed in the proper vectors. These data further support the potential of this hairpin ribozyme as a therapeutic agent for HIV-1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=46924Documentos Relacionados
- Inhibition of human immunodeficiency virus type 1 expression by a hairpin ribozyme.
- Efficient expression by an alphavirus replicon of a functional ribozyme targeted to human immunodeficiency virus type 1.
- Thalidomide inhibits the replication of human immunodeficiency virus type 1.
- Role of the DIS hairpin in replication of human immunodeficiency virus type 1.
- Intracellular immunization of human fetal cord blood stem/progenitor cells with a ribozyme against human immunodeficiency virus type 1.
