A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

AUTOR(ES)

Angelini, Leticia Mara Lima, Silva, Amanda Ribeiro Martins da, Rocco, Lucas de Freitas Coli, Milagre, Cintia Duarte de Freitas

FONTE

Braz. J. Microbiol.

DATA DE PUBLICAÇÃO

31/03/2015

RESUMO

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

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