A large internal deletion converts yeast LEU3 to a constitutive transcriptional activator.
AUTOR(ES)
Friden, P
RESUMO
LEU3 of Saccharomyces cerevisiae encodes an 886-amino-acid polypeptide that activates transcription of at least five genes by binding to an upstream decanucleotide sequence. This activation is dependent on the inducer alpha-isopropylmalate, the synthesis of which is repressed by leucine. We created a 285-amino-acid LEU3 derivative by removing a large block of internal sequences, including a dense cluster of acidic residues. This deletion protein bound to the decanucleotide sequence in vitro and activated gene expression in vivo. In contrast to wild-type LEU3, the truncated LEU3 protein was an effective transcriptional activator when alpha-isopropylmalate synthesis was repressed by leucine.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=362471Documentos Relacionados
- Transcriptional regulator Leu3 of Saccharomyces cerevisiae: separation of activator and repressor functions.
- Manipulation of the 'zinc cluster' region of transcriptional activator LEU3 by site-directed mutagenesis.
- Analysis of constitutive and noninducible mutations of the PUT3 transcriptional activator.
- Structure of yeast regulatory gene LEU3 and evidence that LEU3 itself is under general amino acid control.
- Cloning, Disruption and Chromosomal Mapping of Yeast LEU3 , a Putative Regulatory Gene
