A mechanosensitive ion channel in Schizosaccharomyces pombe.

AUTOR(ES)

Zhou, X L

RESUMO

Protoplast protuberances (blebs) of Schizosaccharomyces pombe were examined using the patch-clamp technique. In addition to several voltage-gated ion channels, we encountered the activities of a mechanosensitive ion channel with a conductance of 180 pS. Microscopic currents of one or two units were observed in some excised patches and ensemble currents of several tens of units were observed in all blebs examined in whole-bleb configuration. This channel opens at pressures of cm Hg applied to whole blebs and it passes cations, including Ca2+. It is inactivated by membrane depolarizations and blocked by Gd3+. We discuss the possible functions of such a channel, including its activation upon cell cycle dependent cytoskeletal reorganizations.

Documentos Relacionados

• Malate transport in Schizosaccharomyces pombe.
• Conjugation-induced lysis of Schizosaccharomyces pombe.
• PCR-mediated direct gene disruption in Schizosaccharomyces pombe.
• Human p53 inhibits growth in Schizosaccharomyces pombe.
• Identification of a GTPase-activating protein homolog in Schizosaccharomyces pombe.