A Mutation in the 5′ Untranslated Region Increases Stability of norA mRNA, Encoding a Multidrug Resistance Transporter of Staphylococcus aureus

AUTOR(ES)

Fournier, Bénédicte

FONTE

American Society for Microbiology

RESUMO

NorA, a multidrug efflux pump in Staphylococcus aureus, protects the cell from multiple drugs, including quinolones. The flqB mutation (T→G) in the 5′ untranslated region upstream of norA causes norA overexpression of 4.9-fold in cis, as measured in norA::blaZ fusions. The transcriptional initiation site of norA was unchanged in mutant and wild-type strains, but the half-life of norA mRNA was increased 4.8-fold in the flqB mutant compared to the wild-type strain. Computer-generated folding of the first 68 nucleotides of the norA transcript predicts an additional stem-loop and changes in a putative RNase III cleavage site in the flqB mutant.

Documentos Relacionados

• Fluoroquinolone resistance protein NorA of Staphylococcus aureus is a multidrug efflux transporter.
• Inhibition of the multidrug transporter NorA prevents emergence of norfloxacin resistance in Staphylococcus aureus.
• Multiple Novel Inhibitors of the NorA Multidrug Transporter of Staphylococcus aureus
• Evidence for the Existence of a Multidrug Efflux Transporter Distinct from NorA in Staphylococcus aureus
• Expression of the Multidrug Resistance Transporter NorA from Staphylococcus aureus Is Modified by a Two-Component Regulatory System