A New Form of DNA Polymerase III and a Copolymerase Replicate a Long, Single-Stranded Primer-Template†
AUTOR(ES)
Wickner, William
RESUMO
A new form of DNA polymerase III, termed Pol III star (Pol III*), has been purified to homogeneity from Escherichia coli. Pol III* is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III. Pol III* and Pol III are separable by gel filtration. Pol III* utilizes a duplex template containing short gaps with the same catalytic properties as Pol III. However, Pol III* is able to replicate long, singlestranded templates such as homopolymer chains and viral circles of M13 and ϕX174 if provided with the following: spermidine, a primer fragment, and a new protein, termed copolymerase III* (Copol III*). The latter, purified to homogeneity, has no known independent enzymatic activity and supports synthesis by Pol III* but not by Pol I, Pol II, or Pol III.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433591Documentos Relacionados
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