A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction
AUTOR(ES)
Fernandes, Aline R., Souza, Paulo S. de, Oliveira, Anselmo E. de, Chaves, Andréa R.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2018-04
RESUMO
A method based on disposable pipette extraction (DPX) was successfully applied to creatinine determination in urine samples analysis using liquid chromatography with ultraviolet spectrophotometric detection (DPX/LC-UV). DPX variables, number of draw/eject cycles, sample pH, and type of the desorption solvent, were employed in a factorial experimental design to optimize the sorption equilibrium and time analysis. Among the evaluated DPX variables, the highest extraction efficiency was obtained with 500 µL of urine sample mixed with 1 mL of borate solution (pH 9) with one draw/eject cycle followed by liquid desorption of 1 mL of methanol in seven draw/eject cycles. The developed DPX/LC-UV method showed a linear response from the limit of quantification of 0.317 to 3.390 g L-1 with r2 = 0.996 and inter-day precision with a coefficient of variation below 8.8%. Based on these results, the proposed method can be a useful tool for determining the creatinine levels in urine samples.
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