A new selective medium for isolating Listeria spp. from heavily contaminated material.

AUTOR(ES)

Bannerman, E S

RESUMO

Food-associated outbreaks of human listeriosis have emphasized the importance and necessity of screening food for the presence of Listeria isolates-selective agar medium combining acriflavine (10 mg/liter) with ceftazidime (50 mg/liter) was developed. A total of 1,099 cheese production specimens were cultured, from which 157 Listeria isolates. (14.3%) grew. When compared with modified McBride agar, the acriflavine-ceftazidime agar recovered more Listeria isolates (98 versus 65%, P less than 0.001) more rapidly (57% after 48 h of incubation of the enrichment broth versus 35%, P less than 0.01) and in greater amounts. Acriflavine-ceftazidime selective agar medium proved to be a highly sensitive medium to recover Listeria spp. from heavily contaminated food products.

Documentos Relacionados

• A new selective medium for isolating Pseudomonas spp. from water.
• Selective diagnostic medium for pathogenic Listeria spp.
• A New Selective Medium for Bifidobacterium spp.
• New methodology for the isolation of Listeria microorganisms from heavily contaminated environments.
• New selective medium for isolating Clostridium difficile from faeces.