A novel p34cdc2-binding and activating protein that is necessary and sufficient to trigger G2/M progression in Xenopus oocytes
AUTOR(ES)
Ferby, Ingvar
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
The activation of maturation-promoting factor (MPF) is required for G2/M progression in eukaryotic cells. Xenopus oocytes are arrested in G2 and are induced to enter M phase of meiosis by progesterone stimulation. This process is known as meiotic maturation and requires the translation of specific maternal mRNAs stored in the oocytes. We have used an expression cloning strategy to functionally identify proteins involved in G2/M progression in Xenopus oocytes. Here we report the cloning of two novel cDNAs that when expressed in oocytes induce meiotic maturation efficiently. The two cDNAs encode proteins of 33 kD that are 88% identical and have no significant homologies to other sequences in databases. These proteins, which we refer to as p33ringo (rapid inducer of G2/M progression in oocytes), induce very rapid MPF activation in cycloheximide-treated oocytes. Conversely, ablation of endogenous p33ringo mRNAs using antisense oligonucleotides inhibits progesterone-induced maturation, suggesting that synthesis of p33ringo is required for this process. We also show that p33ringo binds to and activates the kinase activity of p34cdc2 but does not associate with p34cdc2/cyclin B complexes. Our results identify a novel p34cdc2 binding and activating protein that regulates the G2/M transition during oocyte maturation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=316955Documentos Relacionados
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