A novel, plasmid-based system for studying gene rearrangements in mammalian cells.

AUTOR(ES)

Krauss, R S

RESUMO

We have developed a plasmid-based system for isolating gene rearrangements in mammalian cells by selection for reversion of a promoterless drug resistance gene. pNH4 contains the selectable marker gene neo under the control of the herpes simplex virus, thymidine kinase (tk) promoter and, upstream and in the opposite orientation, a dormant promoterless hygromycin B resistance gene (hph) that can be expressed following rearrangement events. An NIH 3T3 cell line stably transfected with pNH4 that has a spontaneous frequency of generation of Hphr colonies of approximately 10(-8) was isolated. Treatment of this line with ethyl methanesulfonate raised the frequency of Hphr colony formation approximately 100-fold. Approximately 60% (21 of 35) of ethyl methanesulfonate-induced Hphr clones showed rearrangements detectable by Southern blot analysis within a 40-kb region surrounding the integrated construct, including a nonhomologous recombination event and, possibly, a large insertion. Additionally, three Hphr clones showed evidence of gene amplification. Northern (RNA) blot analysis of hph mRNA suggests that the rearrangements may provide a function that allows the tk promoter to initiate transcription off the opposite strand, thus yielding hph transcripts. Cell lines harboring pNH4, or modifications of it, may be valuable for studying recombination mechanisms responsible for the various types of genetic rearrangements found in cancer cells.

Documentos Relacionados

• A plasmid-based system for expressing small interfering RNA libraries in mammalian cells
• Human cytomegalovirus plasmid-based amplicon vector system for gene therapy
• A plasmid-based reverse genetics system for influenza A virus.
• Identification of gene function and functional pathways by systemic plasmid-based ribozyme targeting in adult mice
• Preferential cleavage of plasmid-based R-loops and D-loops by Drosophila topoisomerase IIIβ