A novel yeast protein influencing the response of RNA polymerase II to transcriptional activators
AUTOR(ES)
Emili, Andrew
FONTE
The National Academy of Sciences
RESUMO
A sensitive in vitro crosslinking technique using a photoactive derivative of the chimeric activator LexA-E2F-1 was used to identify yeast proteins that might influence the response of RNA polymerase II to transcriptional activators. We found that a novel yeast protein, Xtc1p, could be covalently crosslinked to the activation domain of LexA-E2F-1 when this derivatized activator was bound to DNA upstream of an activator-responsive RNA polymerase II promoter. Because affinity chromatography experiments showed that Xtc1p also bound directly and specifically to the activation domains of E2F-1, the viral activator VP16, and the yeast activator Gal4p and copurified with the RNA polymerase II holoenzyme complex, Xtc1p may modulate the response of RNA polymerase II to multiple activators. Consistent with this notion, yeast strains deleted for the XTC1 gene exhibited pleiotropic growth defects, including temperature sensitivity, galactose auxotrophy, and a heightened sensitivity to activator overexpression, as well as an altered response to transcriptional activators in vivo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=21606Documentos Relacionados
- Transcriptional arrest of yeast RNA polymerase II by Escherichia coli rho protein in vitro.
- Yeast GCN4 transcriptional activator protein interacts with RNA polymerase II in vitro.
- A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans.
- Transcriptional terminators of RNA polymerase II are associated with yeast replication origins.
- Activator-Specific Requirement of Yeast Mediator Proteins for RNA Polymerase II Transcriptional Activation
