A nuclear single-stranded-DNA binding factor interacts with the long terminal repeats of the 1731 Drosophila retrotransposon.

AUTOR(ES)

Fourcade-Peronnet, F

RESUMO

Using gel mobility assays, we have detected two proteins that bind in the U3 region of the 1731 retrotransposon long terminal repeats (between positions -110 and -73) in nuclear extracts from Drosophila melanogaster cultured cells. The first one binds double-stranded DNA, whereas the other binds the mRNA-like strand in a sequence-specific manner. We report here the characterization of the latter protein, named NssBF for nuclear single-stranded-DNA binding factor. Gel filtration shows an apparent molecular mass of 95 kDa for NssBF. The points of contact between NssBF and its single-stranded DNA target were determined. This protein binds neither the complementary strand nor the corresponding RNA sequence. A possible role of NssBF in transcription is discussed.

Documentos Relacionados

• Structural flexibility of a DNA hairpin located in the long terminal repeat of the Drosophila 1731 retrotransposon.
• Identification and Characterization of the Fourth Single-Stranded-DNA Binding Domain of Replication Protein A
• Replication origins of single-stranded-DNA plasmid pUB110.
• Single-stranded-DNA binding alters human replication protein A structure and facilitates interaction with DNA-dependent protein kinase.
• Functional analysis of the long terminal repeats of Drosophila 1731 retrotransposon: promoter function and steroid regulation.