A Nucleic Acid Associated with a Killer Strain of Yeast
AUTOR(ES)
Vodkin, Michael H.
RESUMO
A crude membrane fraction was isolated from both killer M(k) and isogenic nonkiller M(o) strains of yeast labeled with [3H]adenine. Nucleic acids were extracted from this fraction and centrifuged to equilibrium in an ethidium bromide-Cs2SO4 solution. A peak of radioactivity coincident with a single fluorescent band was present in the membrane fraction isolated from cells of the killer strain but was absent from that isolated from nonkiller cells. This material has been characterized as double-stranded RNA by treatment with various nucleases and by chromatography on a cellulose CF-11 column. Sedimentation in a sucrose gradient indicates an S value of 8-10.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433427Documentos Relacionados
- Construction of Killer Wine Yeast Strain
- Yeast Killer Mutants with Altered Double-Stranded Ribonucleic Acid
- Determination of killer yeast activity in fermenting grape juice by using a marked Saccharomyces wine yeast strain.
- Metabolic changes associated with the germination of corn. II. Nucleic acid metabolism.
- Characterization and classification of virus particles associated with hepatitis A. II. Type and configuration of nucleic acid.
