A phorbol ester/diacylglycerol-binding protein encoded by the unc-13 gene of Caenorhabditis elegans.
AUTOR(ES)
Maruyama, I N
RESUMO
Mutations in the unc-13 gene cause diverse defects in the nervous system of the nematode Caenorhabditis elegans. Molecular cloning of the gene and sequencing of the cDNA revealed that the product encodes a protein, 1734 amino acids in length, with a central domain with sequence similarity to the regulatory region of protein kinase C. The domain was expressed in Escherichia coli and shown to bind specifically to a phorbol ester in the presence of calcium; diacylglycerol inhibited the binding in a competitive manner. These findings confirm that the unc-13 gene product has binding sites similar to those of protein kinase C and may be a component of an alternative transduction pathway of the diacylglycerol signal to a different effector function in the nervous system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=51951Documentos Relacionados
- Expression of Multiple UNC-13 Proteins in the Caenorhabditis elegans Nervous System
- Competitive inhibition by diacylglycerol of specific phorbol ester binding.
- Additional Sequence Complexity in the Muscle Gene, Unc-22, and Its Encoded Protein, Twitchin, of Caenorhabditis Elegans
- Suppressors of the Unc-73 Gene of Caenorhabditis Elegans
- The genome of Caenorhabditis elegans.