A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration.

AUTOR(ES)
RESUMO

Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.

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