A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5'-terminal cap in mRNA.

AUTOR(ES)

Sonenberg, N

RESUMO

Protein synthesis initiation factors prepared from rabbit reticulocyte and mouse ascites ribosomes were tested for the ability to crosslink to the 5' cap of mRNA. Crosslinking of one polypeptide of apparent molecular weight 24,000 was inhibited by the cap analogs, m7GMP and m7GDP, indicating a specific interaction with the cap. Although specific crosslinking of the 24,000 molecular weight polypeptide was found with eukaryotic initiation factor 3 and to a lesser extent with initiation factor 4B, both of these factors contained less than stoichiometric amounts of this polypeptide. The crosslinking method provides a highly sensitive and specific assay for cap-binding proteins and should facilitate their purification for functional studies.

Documentos Relacionados

• Characterization of the 5'-terminal cap structures of early simian virus 40 mRNA.
• Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.
• Recognition of 5′-terminal TAR structure in human immunodeficiency virus-1 mRNA by eukaryotic translation initiation factor 2
• Transfer of 5′-terminal cap of globin mRNA to influenza viral complementary RNA during transcription in vitro
• On the base-stacking in the 5'-terminal cap structure of mRNA: a fluorescence study.