A Potato Virus X Resistance Gene Mediates an Induced, Nonspecific Resistance in Protoplasts.
AUTOR(ES)
Kohm, BA
RESUMO
The Rx locus in potato controls extreme resistance to most isolates of potato virus X (PVX). The resistance is expressed in whole plants and in protoplasts. Rx-mediated resistance in protoplasts causes reduced accumulation of all PVX RNA species, including the (-) strand RNA after a lag of 8 hr postinoculation. In work reported elsewhere, we have shown that the Rx-breaking property of PVXHB was associated with the coat protein gene of PVXUK3 and PVXCP4. Here, we describe how a frameshift mutation in the coat protein gene had no effect on Rx resistance breaking but compromised the Rx-mediated resistance to PVXCP4. We also describe how in coinoculation experiments, the Rx-mediated resistance could be induced to affect PVXHB or cucumber mosaic virus (CMV). In these experiments, PVXHB or CMV was coinoculated to protoplasts (Rx genotype) together with an isolate of PVX, which is affected by Rx. We interpret this data to indicate that Rx-mediated resistance is induced when the PVX coat protein is produced in the infected cells and that the induced resistance mechanism is effective against viruses unrelated to PVX.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=160326Documentos Relacionados
- Potato Virus X-Induced Gene Silencing in Leaves and Tubers of Potato1
- Mutanolysin-induced spheroplasts of Streptococcus mutants are true protoplasts.
- Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.
- Methylation of coding region alone inhibits gene expression in plant protoplasts.
- Thiamine transport in Saccharomyces cerevisiae protoplasts.
