A rapid and simple method to detect ESBL in Enterobacter cloacae based on MIC of cefepime

AUTOR(ES)

Bastos, Marina de Souza, Menegucci, Thatiany Cevallos, Moreira, Rafael Renato Brondani, Garcia, Lourdes Botelho, Cardoso, Celso Luiz, Tognim, Maria Cristina Bronharo

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

2015-04

RESUMO

INTRODUCTION: The aim of this study was to identify a rapid and simple phenotypic method for extended-spectrum β-lactamase (ESBL) detection in Enterobacter cloacae. METHODS: A total of 79 consecutive, non-repeated samples of E. cloacae were evaluated. Four phenotypic methods were applied for ESBL detection, results were compared to multiplex polymerase chain reaction (PCR) as the gold standard reference method: 1) ceftazidime and cefotaxime disks with and without clavulanate, both with boronic acid added; 2) disk approximation using cefepime and amoxicillin/clavulanate; 3) ESBL screening by minimum inhibitory concentration (MIC) ≥ 16µg/mL and 4) by MIC ≥ 2µg/mL for cefepime. RESULTS: Method 4 showed the best combination of sensitivity (100%) and specificity (94%). CONCLUSIONS: MIC ≥ 2µg/mL for cefepime would be very useful for the phenotypic detection of ESBL in samples of E. cloacae.

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