A Refined Map of the hisG Gene of SALMONELLA TYPHIMURIUM
AUTOR(ES)
Hoppe, I.
RESUMO
The hisG gene is the most operator-proximal structural gene of the histidine operon; it encodes the feedback-inhibitable first enzyme of the biosynthetic pathway. Previously, hisG mutants were mapped into seven intervals defined by the available deletion mutations having endpoints in the hisG gene. The map has been refined using over 60 new deletion mutants. The new map divides the gene into 40 deletion intervals, which average approximately 30 base pairs in length. The map has been used to analyze the distribution of insertion sites for the transposable element Tn10 and has permitted conclusions on the distribution of duplication endpoints. The map promises to be useful in analysis of his regulation and, more particularly, in the determination of the possible role of the hisG enzyme in this mechanism.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1213939Documentos Relacionados
- Deletions fusing the hisG and hisD genes in Salmonella typhimurium.
- Features of the rho-dependent transcription termination polar element within the hisG cistron of Salmonella typhimurium.
- Histidine Mutants Requiring Adenine: Selection of Mutants with Reduced hisG Expression in SALMONELLA TYPHIMURIUM
- Salmonella typhimurium hisG46 (R-Utrecht): Possible Use in Screening Mutagens and Carcinogens
- Regulation of histidine operon does not require hisG enzyme.