A Resource of Mapped Dissociation Launch Pads for Targeted Insertional Mutagenesis in the Arabidopsis Genome1
AUTOR(ES)
Muskett, Paul R.
FONTE
The American Society for Plant Biologists
RESUMO
We describe a new resource for targeted insertional mutagenesis in Arabidopsis using a maize (Zea mays) Activator/Dissociation (Ds) two-element system. The two components of the system, T-DNA vectors carrying a Ds launch pad and a stable Activator transposase source, were designed to simplify selection of transposition events and maximize their usefulness. Because Ds elements preferentially transpose to nearby genomic sites, they can be used in targeted mutagenesis of linked genes. To efficiently target all genes throughout the genome, we generated a large population of transgenic Arabidopsis plants containing the Ds launch pad construct, identified lines containing single Ds launch pad inserts, and mapped the positions of Ds launch pads in 89 lines. The integration sites of the Ds launch pads were relatively evenly distributed on all five chromosomes, except for a region of chromosomes 2 and 4 and the centromeric regions. This resource therefore provides access to the majority of the Arabidopsis genome for targeted tagging.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=166993Documentos Relacionados
- Annotation of the Arabidopsis Genome1
- The Organization of Cytoplasmic Ribosomal Protein Genes in the Arabidopsis Genome1
- A New Resource of Locally Transposed Dissociation Elements for Screening Gene-Knockout Lines in Silico on the Arabidopsis Genome1[w]
- Maize-targeted mutagenesis: A knockout resource for maize
- Site Preferences of Insertional Mutagenesis Agents in Arabidopsis