A second global regulator gene (arcB) mediating repression of enzymes in aerobic pathways of Escherichia coli.
AUTOR(ES)
Iuchi, S
RESUMO
In Escherichia coli anaerobic growth lowers the basal or induced levels of numerous enzymes associated with aerobic metabolism. Mutations in arcA (dye) at min 0 relieve this pleiotropic anaerobic repression and render the cell sensitive to the redox dye toluidine blue. In this study we identified a second pleiotropic control gene, arcB, at min 69.5. Mutations, including a deletion, in this gene also relieved the anaerobic repression and caused sensitivity to toluidine blue. Mutations in arcA or arcB did not significantly change the catabolite repression of the target phi(sdh-lacZ) operon, in which lacZ is fused to a structural gene for succinate dehydrogenase, nor did the mutations strikingly influence the pattern of excretion products during glucose fermentation. The presence of arcA+ in a multicopy plasmid restored anaerobic repression in arcB mutants, as indicated by the expression of phi(sdh-lacZ). The arcB product might be a sensor protein for the redox or energy state of the arc regulatory system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=209676Documentos Relacionados
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