A sedimentation study of the interaction of superhelical SV40 DNA with H1 histone.
AUTOR(ES)
Böttger, M
RESUMO
By moving boundary sedimentation it is shown that the interaction of H1 histone with superhelical circular SV40 DNA results in the formation of giant heterogeneous aggregates. The size of these aggregates grows with increasing H1 concentration. s20,w values of some 10 000 S were measured. As compared with open relaxed circular DNA a preferential interaction of superhelical DNA with H1 histone is observed, irrespective of the sign of the superhelical turns which was reversed by the addition to DNA of ethidium bromide. The addition to the H1 complexed aggregates of ethidium bromide effects a progressive breakdown of the aggregates. Furthermore, the superhelicity of DNA is not changed by the addition of small amounts of H1 histone.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342912Documentos Relacionados
- Interaction of histone H1 with superhelical DNA. Sedimentation and electron microscopical studies at low salt concentration.
- Studies on the interaction of H1 histone with superhelical DNA: characterization of the recognition and binding regions of H1 histones.
- Chromosome of the mature virion of simian virus 40 contains H1 histone.
- Cooperative interaction of histone H1 with DNA.
- Specific interaction of histone H1 with eukaryotic DNA.
