A short 5' region of the long terminal repeat is required for regulation by hormone and heat shock of Drosophila retrotransposon 1731.

AUTOR(ES)

Ziarczyk, P

RESUMO

1731, a Drosophila retrotransposon was first described as having a transcription activity which was negatively regulated by 20-hydroxyecdysone (20-OH), the steroid molting hormone of insects. Using constructions expressing the bacterial chloramphenicol-acetyltransferase (CAT) gene under the control of the entire or deleted Long Terminal Repeats (LTRs) of 1731, we were able to show that a short (28 bp) sequence located in the U3 region of these LTRs was required for 1) the increase in promoter strength, 2) negative regulation by 20-OH and, 3) positive regulation by heat shock.

Documentos Relacionados

• Structural flexibility of a DNA hairpin located in the long terminal repeat of the Drosophila 1731 retrotransposon.
• Characterization and cloning of p11, a transrepressor of Drosophila melanogaster retrotransposon 1731.
• Functional analysis of the long terminal repeats of Drosophila 1731 retrotransposon: promoter function and steroid regulation.
• The 5′ Untranslated Region and Gag product of Idefix, a Long Terminal Repeat-Retrotransposon from Drosophila melanogaster, Act Together To Initiate a Switch between Translated and Untranslated States of the Genomic mRNA
• A nuclear single-stranded-DNA binding factor interacts with the long terminal repeats of the 1731 Drosophila retrotransposon.