A short sequence in the N-terminal region is required for the trimerization of type XIII collagen and is conserved in other collagenous transmembrane proteins
AUTOR(ES)
Snellman, Anne
FONTE
Oxford University Press
RESUMO
The recombinant transmembrane protein type XIII collagen is shown to reside on the plasma membrane of insect cells in a ‘type II’ orientation. Expressions of deletion constructs showed that sequences important for the association of three α1(XIII) chains reside in their N- rather than C-terminal portion. In particular, a deletion of residues 63–83 immediately adjacent to the transmembrane domain abolished the formation of disulfide-bonded trimers. The results imply that nucleation of the type XIII collagen triple helix occurs at the N-terminal region and that triple helix formation proceeds from the N- to the C-terminus, in opposite orientation to that of the fibrillar collagens. Interestingly, a sequence homologous to the deleted residues was found at the same plasma membrane-adjacent location in other collagenous transmembrane proteins, suggesting that it may be a conserved association domain. The type XIII collagen was secreted into insect cell medium in low amounts, but this secretion was markedly enhanced when the cytosolic portion was lacking. The cleavage occurred in the non-collagenous NC1 domain after four arginines and was inhibited by a furin protease inhibitor.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=302104Documentos Relacionados
- The conserved N-terminal region of Sendai virus nucleocapsid protein NP is required for nucleocapsid assembly.
- Abnormal adherence junctions in the heart and reduced angiogenesis in transgenic mice overexpressing mutant type XIII collagen
- Identification of a Conserved N-Terminal Sequence Involved in Transmembrane Signal Transduction in EnvZ
- A C-terminal domain conserved in precursor processing proteases is required for intramolecular N-terminal maturation of pro-Kex2 protease.
- The N-Terminal Region of the Escherichia coli WecA (Rfe) Protein, Containing Three Predicted Transmembrane Helices, Is Required for Function but Not for Membrane Insertion