A simple method for human peripheral blood monocyte Isolation

AUTOR(ES)

Almeida, Marcos C de, Silva, Alan C, Barral, Aldina, Barral Netto, Manoel

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2000-04

RESUMO

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.

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