A Simple, Rapid Method for Enumerating Total Viable and Metabolically Active Bacteria in Groundwater
AUTOR(ES)
King, Laura K.
RESUMO
We report here a new staining procedure which uses both the enzymatic dehydrogenation of 2-(p-iodophenyl)-3-p-(nitrophenyl)-5 phenyltetrazolium chloride to a pink intracellular formazan and the DNA-specific fluorochrome 4′,6′-diamidino-2-phenylindole. Application of this staining procedure to cells concentrated on filters and then transferred to microscope slides by the filter-transfer-freeze technique has proven valuable for statistically accurate enumeration of the total viable and metabolically active cells in groundwaters.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202712Documentos Relacionados
- A Simple, Rapid Method for Demonstrating Bacterial Flagella
- A SIMPLE, RAPID METHOD FOR DETERMINATION OF LEAF CAROTENE
- A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
- A simple, rapid ELISA method for the detection of DNA antibodies.
- Rickettsial hemolysis: rapid method for enumeration of metabolically active typhus rickettsiae.
