A simplified system for generating recombinant adenoviruses
AUTOR(ES)
He, Tong-Chuan
FONTE
The National Academy of Sciences
RESUMO
Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=19394Documentos Relacionados
- Simplified restriction endonuclease method for typing and subtyping adenoviruses.
- A simplified method of generating transgenic Xenopus
- A NEW SIMPLIFIED SYSTEM FOR INTRAOPERATIVE COUNTERPULSATION
- A MECHANISM FOR GENETIC RECOMBINATION GENERATING ONE PARENT AND ONE RECOMBINANT*
- A simplified prognostic system for resected pancreatic neuroendocrine neoplasms
