A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
AUTOR(ES)
Gallo, Stephanie Wagner, Ramos, Patricia Locosque, Ferreira, Carlos Alexandre Sanchez, Oliveira, Silvia Dias de
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
2013-05
RESUMO
Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
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