A study of folate absorption and metabolism in man utilizing carbon-14—labeled polyglutamates synthesized by the solid phase method
AUTOR(ES)
Butterworth, C. E.
RESUMO
The absorption and metabolism of synthetic polyglutamates of folic acid have been compared with free pteroylglutamic acid in four subjects having chronic lymphatic leukemia and one with Hodgkin's granuloma. Pteroylpolyglutamates containing either three or seven glutamate residues were prepared by the solid-phase method permitting placement of carbon-14 labels in either the pteridine ring or in a selected glutamate unit of the gamma peptide chain. Complete dissociation was observed between biological folate activity and radioactivity of plasma after ingestion of pteroyltriglutamate labeled in the middle glutamate. This indicates cleavage to the monoglutamate form at the time of absorption from the intestine or very soon thereafter. A large portion of radioactivity liberated from the middle glutamate is recoverable as carbon dioxide in the exhaled air.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=322328Documentos Relacionados
- Metabolism of Carbon-14-Labeled Benzene and Tolueue in Avocado Fruit
- Conversion of Carbon-14-Labeled Urea into Amino Acids in Leaves. 12
- Isolation of Carbon-14-labeled Sedoheptulose and Other Products from Sedum spectabile. 12
- The Biosynthesis of Carbon-14-Labeled Compounds. III. The Separation and Isolation of Sugars by Ion-exchange Chromatography1
- THE BIOSYNTHESIS OF CARBON-14-LABELED COMPOUNDS. I. THE CHROMATOGRAPHIC SEPARATION OF GLUCOSE AND FRUCTOSE 1