A Synergistic Stimulation of Avena sativa Coleoptile Elongation by Indoleacetic Acid and Carbon Dioxide 1

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The ability of 0.03% CO2 to stimulate growth has been investigated using etiolated Avena coleoptile sections maintained in buffered solution. This concentration of CO2-stimulated growth after a lag period of 12 to 15 minutes, and a synergistic relationship between indoleacetic acid and CO2 in stimulating growth has been demonstrated. The response to CO2 is inhibited by cycloheximide and is lost approximately 10 minutes after exposure to CO2-free air. Malate can replace CO2 in stimulating growth. In the light of these data and recent literature on a growth response of coleoptile sections to CO2-saturated solutions, the existence of two mechanisms of CO2-simulated growth is proposed. In addition, it is suggested that growth promotion by 0.03% CO2 is mediated by a process involving dark CO2 fixation.

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