A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene.
AUTOR(ES)
Pelham, H R
RESUMO
Previous deletion analysis of the Drosophila hsp70 heat-shock promoter has identified a sequence upstream of the TATA box that is required for heat induction. This region contains homology to other heat-shock promoters, and it was proposed that the common sequence is an important element in the regulation of the heat-shock genes. We have constructed sequences similar to the consensus CT-GAA-TTC-AG from synthetic oligonucleotides and placed them upstream of the TATA box of the herpes virus thymidine kinase gene, in place of the normal upstream promoter element. The resultant genes are heat-inducible both in monkey COS cells and in Xenopus oocytes. We conclude that the transcriptional heat-shock response is mediated by some factor that interacts with this sequence.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553234Documentos Relacionados
- Herpes simplex virus infection causes the accumulation of a heat-shock protein.
- The Heat-Shock Element Is a Functional Component of the Arabidopsis APX1 Gene Promoter1
- Evidence for protection by heat-shock proteins against photoinhibition during heat-shock
- In situ detection of a heat-shock regulatory element binding protein using a soluble synthetic enhancer sequence.
- Archaebacterial heat-shock proteins
