A synthetic peptide substrate specific for casein kinase II.
AUTOR(ES)
Kuenzel, E A
RESUMO
A synthetic peptide having the sequence Arg-Arg-Arg-Glu-Glu-Thr-Glu-Glu-Glu was found to serve as a convenient substrate for the protein kinase generally referred to as casein kinase II. The enzyme exhibited an apparent Km of 500 microM for the peptide, as compared to an apparent Km of 50 microM for casein. The maximum velocities for phosphorylation of the peptide and of casein were similar. The peptide was not phosphorylated by any of eight other protein kinases, all of which were shown to be active toward their known substrates. The peptide was used to monitor activity during steps in the purification of casein kinase II from bovine liver. These experiments demonstrated that with this peptide it is now possible to obtain specific measurements of casein kinase II activity in crude enzyme preparations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=397121Documentos Relacionados
- Myc oncoproteins are phosphorylated by casein kinase II.
- Constitutive phosphorylation of I kappa B alpha by casein kinase II.
- Activation of mammalian DNA ligase I through phosphorylation by casein kinase II.
- In vivo phosphorylation of a synthetic peptide substrate of cyclic AMP-dependent protein kinase.
- Amino acid sequence of the beta subunit of bovine lung casein kinase II.