A system for tissue-specific gene targeting: transgenic mice susceptible to subgroup A avian leukosis virus-based retroviral vectors.
AUTOR(ES)
Federspiel, M J
RESUMO
Avian leukosis viruses (ALVs) have been used extensively as genetic vectors in avian systems, but their utility in mammals or mammalian cell lines is compromised by inefficient viral entry. We have overcome this limitation by generating transgenic mice that express the receptor for the subgroup A ALV under the control of the chicken alpha sk-actin promoter. The skeletal muscles of these transgenic animals are susceptible to efficient infection by subgroup A ALV. Because infection is restricted to cell lineages that express the transgene, the method has utility for studies of development and oncogenesis and will provide models for tissue-specific gene therapy.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=45203Documentos Relacionados
- Expression of transduced genes in mice generated by infecting blastocysts with avian leukosis virus-based retroviral vectors.
- Generation of a helper cell line for packaging avian leukosis virus-based vectors.
- Packaging cells for avian leukosis virus-based vectors with various host ranges.
- Psi- vectors: murine leukemia virus-based self-inactivating and self-activating retroviral vectors.
- An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells
