A tentative model of the intercalative binding of the neocarzinostatin chromophore to double-stranded tetranucleotides.

AUTOR(ES)

Chen, K X

RESUMO

Theoretical computations are performed of the intercalative binding of the neocarzinostatin chromophore (NCS) with the double-stranded oligonucleotides d(CGCG)2, d(GCGC)2, d(TATA)2 and d(ATAT)2. Minor groove binding is preferred over major groove binding. It is found that the long axis of the stacked naphtoate ring lies approximately parallel to the long axis of the base pairs of the intercalation site. The galactosamine ammonium group interacts with specific sites of the groove (O2/N3 of bases 2 and O1' of sugar S3), whereas the dodecadyine ring system wraps around the groove towards the backbone. An overall AT versus GC preference is derived. Intercalation in a central purine-(3', 5')-pyrimidine sequence appears to be preferred over that in a central pyrimidine-(3', 5')-purine sequence.

Documentos Relacionados

• A theoretical investigation on the sequence selective binding of mitoxantrone to double-stranded tetranucleotides.
• Drosophila X virus RNA polymerase: tentative model for in vitro replication of the double-stranded virion RNA.
• A conserved double-stranded RNA-binding domain.
• Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase.
• Intercalators promote the binding of RecA protein to double-stranded DNA.