A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing.
AUTOR(ES)
Lewis, J D
RESUMO
The function in splicing of a heterodimeric nuclear cap binding complex (yCBC) from the yeast Saccharomyces cerevisiae has been examined. Immunodepletion of splicing extracts with antibodies directed against one component of the complex, yCBP80, results in the efficient co-depletion of the second component, yCBP20, producing CBC-deficient splicing extract. This extract exhibits strongly reduced splicing efficiency and similar reductions in the assembly of both spliceosomes and of the earliest defined precursors to spliceosomes, commitment complexes. The addition of highly purified yCBC substantially restores these defects. These results, together with other data, suggest that CBCs play a highly conserved role in the recognition of pre-mRNA substrates at an early step in the splicing process.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146107Documentos Relacionados
- The stereochemical course of the first step of pre-mRNA splicing.
- Assembly of pre-mRNA splicing complex is cap dependent.
- Short artificial hairpins sequester splicing signals and inhibit yeast pre-mRNA splicing.
- PRP18, a protein required for the second reaction in pre-mRNA splicing.
- Evidence for a role for galectin-1 in pre-mRNA splicing.
